bcr-abl Translocation for Acute Lymphocytic Leukemia
Whole blood or bone marrow
7 mL EDTA whole blood or 1-2 mL EDTA bone marrow
3 mL whole blood
Lavender-stopper (EDTA) tubes
Submit at room temperature using Leukemia/Lymphoma Specimen Transport Kit (supplied by the Hospital). Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on test request form.
Maintain specimen at room temperature.
Causes for Rejection:
Specimen frozen; specimen in formalin or other fixative; clotted specimen; hemolysis; blood more than 72 hours old; bone marrow aspirates older than 5 days; bags or bottles of body fluid or bronchial washings
Detect Philadelphia chromosome; monitor effectiveness of treatment; detect minimal residual disease
This test should not be selected for CML patients.
Reverse transcriptase polymerase chain reaction (RT-PCR) and allele-specific oligonucleotide (ASO) analysis
Approximately 5% of childhood ALLs and 25% of adult ALLs carry a bcr-abl chromosomal translocation t(9;22) which is associated with poor prognosis.