bcr-abl Translocation for Chronic Myelogenous Leukemia
Whole blood or bone marrow
7 mL whole blood or 1-2 mL bone marrow
3 mL whole blood
Lavender-stopper (EDTA) tubes
Submit at room temperature. Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on test request form.
Maintain specimen at room temperature.
Causes for Rejection:
Specimen frozen; specimen in formalin or other fixative; clotted specimen; hemolysis; blood more than 72 hours old; bone marrow aspirates older than 5 days; bags or bottles of body fluid or bronchial washings
Aid in the diagnosis of chronic myelogenous leukemia (CML) and monitoring for minimal residual disease. CML is associated with a bcr-abl chromosomal translocation t(9; 22) in >95% of all cases.
Reverse transcriptase polymerase chain reaction (RT-PCR) and allele-specific oligonucleotide (ASO) analysis