T-Cell Receptor Gene Rearrangements for Leukemia
DNA probe to the T-cell receptor beta-chain constant region
Whole blood, bone marrow, lymph node, or other tissue
7 mL whole blood or 2 cc bone marrow or 0.5-1 g tissue
3 mL whole blood
Whole blood or bone marrow in lavender-stopper (EDTA) tubes, lymph node or tissue in lymph node transport bottle
Specimen should arrive in the laboratory within 48 hours of collection. Please state the date and time of collection on the test request form.
Maintain specimen at room temperature.
Causes for Rejection:
Specimen frozen; specimen in formalin or other fixative; clotted specimen; hemolysis; blood more than 72 hours old; bone marrow aspirates older than 5 days
Determine the clonality and lineage of suspected neoplastic lymphoid cell populations by detecting gene rearrangements that occur normally during the maturation of lymphoid cells into T-lymphocytes
Southern blot hybridization