The National Health Information Exchange

Services: Cancer : Hematopathology : T-Cell Receptor Gene

T-Cell Receptor Gene Rearrangements for Leukemia

    Test Includes:

    DNA probe to the T-cell receptor beta-chain constant region.

    Special Instructions:

    Whole blood, bone marrow, lymph node, or other tissue.


    7 mL whole blood or 2 cc bone marrow or 0.5-1 g tissue.


    3 mL whole blood.


    Whole blood or bone marrow in lavender-stopper (EDTA) tubes, lymph node or tissue in lymph node transport bottle.


    Specimen should arrive in the laboratory within 48 hours of collection. Please state the date and time of collection on the test request form.


    Maintain specimen at room temperature.

    Causes for Rejection:

    Specimen frozen; specimen in formalin or other fixative; clotted specimen; hemolysis; blood more than 72 hours old; bone marrow aspirates older than 5 days.


    Determine the clonality and lineage of suspected neoplastic lymphoid cell populations by detecting gene rearrangements that occur normally during the maturation of lymphoid cells into T-lymphocytes.


    Southern blot hybridization.