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Services: Cancer : Hematopathology : T-Cell Receptor Gene

T-Cell Receptor Gene Rearrangements for Leukemia

    Test Includes:

    DNA probe to the T-cell receptor beta-chain constant region.

    Special Instructions:

    Whole blood, bone marrow, lymph node, or other tissue.

    Specimen:

    7 mL whole blood or 2 cc bone marrow or 0.5-1 g tissue.

    Volume:

    3 mL whole blood.

    Container:

    Whole blood or bone marrow in lavender-stopper (EDTA) tubes, lymph node or tissue in lymph node transport bottle.

    Collection:

    Specimen should arrive in the laboratory within 48 hours of collection. Please state the date and time of collection on the test request form.

    Storage:

    Maintain specimen at room temperature.

    Causes for Rejection:

    Specimen frozen; specimen in formalin or other fixative; clotted specimen; hemolysis; blood more than 72 hours old; bone marrow aspirates older than 5 days.

    Use:

    Determine the clonality and lineage of suspected neoplastic lymphoid cell populations by detecting gene rearrangements that occur normally during the maturation of lymphoid cells into T-lymphocytes.

    Methodology:

    Southern blot hybridization.