Services: Hematopathology : Bcr-abl Translocation

Bcr-abl Translocation for acute lymphocytic leukemia

    Test Includes:

    Philadelphia Chromosome.

    Specimen:

    Whole blood or bone marrow.

    Volume:

    7 mL EDTA whole blood or 1-2 mL EDTA bone marrow.

    Minimum Volume:

    3 mL whole blood

    Container:

    Lavender-stopper (EDTA) tubes.

    Collection:

    Submit at room temperature using Leukemia/Lymphoma Specimen Transport Kit (supplied by the Hospital). Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on test request form.

    Storage:

    Maintain specimen at room temperature.

    Causes for Rejection:

    Specimen frozen; specimen in formalin or other fixative; clotted specimen; hemolysis; blood more than 72 hours old; bone marrow aspirates older than 5 days; bags or bottles of body fluid or bronchial washings

    Use:

    Detect Philadelphia chromosome; monitor effectiveness of treatment; detect minimal residual disease.

    Contraindications:

    This test should not be selected for CML patients.

    Methodology:

    Reverse transcriptase polymerase chain reaction (RT-PCR) and allele-specific oligonucleotide (ASO) analysis.

    Additional Information:

    Approximately 5% of childhood ALLs and 25% of adult ALLs carry a bcr-abl chromosomal translocation t(9;22) which is associated with poor prognosis.

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