Services: Hematopathology : Bcr-abl Translocation

Bcr-abl Translocation for Chronic Myelogenous Leukemia

    Synonyms:

    Philadelphia Chromosome.

    Specimen:

    Whole blood or bone marrow.

    Volume:

    7 mL whole blood or 1-2 mL bone marrow.

    Minimum Volume:

    3 mL whole blood.

    Container:

    Lavender-stopper (EDTA) tubes.

    Collection:

    Submit at room temperature. Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on test request form.

    Storage:

    Maintain specimen at room temperature.

    Causes for Rejection:

    Specimen frozen; specimen in formalin or other fixative; clotted specimen; hemolysis; blood more than 72 hours old; bone marrow aspirates older than 5 days; bags or bottles of body fluid or bronchial washings.

    Use:

    Aid in the diagnosis of chronic myelogenous leukemia (CML) and monitoring for minimal residual disease. CML is associated with a bcr-abl chromosomal translocation t(9; 22) in >95% of all cases.

    Methodology:

    Reverse transcriptase polymerase chain reaction (RT-PCR) and allele-specific oligonucleotide (ASO) analysis.

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